Messenger RNA processing in Methanocaldococcus (Methanococcus) jannaschii
نویسندگان
چکیده
منابع مشابه
Protein-coding gene promoters in Methanocaldococcus (Methanococcus) jannaschii
Although Methanocaldococcus (Methanococcus) jannaschii was the first archaeon to have its genome sequenced, little is known about the promoters of its protein-coding genes. To expand our knowledge, we have experimentally identified 131 promoters for 107 protein-coding genes in this genome by mapping their transcription start sites. Compared to previously identified promoters, more than half of ...
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We have adapted an electrophoretic mobility shift assay (EMSA) to isolate genomic DNA fragments that bind the archaeal transcription initiation factors TATA-binding protein (TBP) and transcription factor B (TFB) to perform a genome-wide search for promoters. Mobility-shifted fragments were cloned, tested for their ability to compete with known promoter-containing fragments for a limited concent...
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Bacterial cell division depends on the formation of a cytokinetic ring structure, the Z-ring. The bacterial tubulin homologue FtsZ is required for Z-ring formation. FtsZ assembles into various polymeric forms in vitro, indicating a structural role in the septum of bacteria. We have used recombinant FtsZ1 protein from M. jannaschii to produce helical tubes and sheets with high yield using the GT...
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The total genome sequence of the archaeon Methanococcus jannaschii completes the trilogy of genomes for the three domains of life-Archaea, Bacteria and Eucarya. It will have far-reaching consequences for evolutionary studies and for the way in which experimental biology is performed.
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We have identified 141 novel palindromic repetitive elements in the genome of euryarchaeon Methanocaldococcus jannaschii. The total length of these elements is 14.3kb, which corresponds to 0.9% of the total genomic sequence and 6.3% of all extragenic regions. The elements can be divided into three groups (MJRE1-3) based on the sequence similarity. The low sequence identity within each of the gr...
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ژورنال
عنوان ژورنال: RNA
سال: 2009
ISSN: 1355-8382
DOI: 10.1261/rna.1715209